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Colheita e processamento de amostras de sangue em suinos para fins de diagnostico. Infoteca-e
MORENO, A. M.; SOBESTIANSKY, J.; LOPEZ, A. C.; SOBESTIANSKY, A. A. B..
Objetivos da colheita de sangue em suinos; Caracteristicas da amostra; Principais anticoagulantes; Material necessario para colheita de sangue; Material; Identificacao de amostras; Colheita de sangue; Contencao do suino de acordo com a idade; Vias de acesso ao sangue; Veia cava cranial; Veia jugular; Veia cefalica; Vasos da orelha; Vasos da cauda; Seio orbital; Comparacao entre as tecnicas de colheita de sangue; Sequencia indicada para colheita de sangue por puncao venosa; Processamento de amostra; Armanezamento e viabilidade do sangue; Valores sanguineos de referencia; Tipos sanguineos dos suinos.
Tipo: Documentos (INFOTECA-E) Palavras-chave: Coleta.; Sangue; Suíno.; Blood; Swine..
Ano: 1997 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/433735
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Características hematológicas de teleósteos brasileiros: II. Parâmetros sangüíneos do Piaractus mesopotamicus Holmberg (Osteichthyes, Characidae) em policultivo intensivo Zoologia
Tavares-Dias,Marcos; Tenani,Rosilene A; Gioli,Leonardo D; Faustino,Christiane D.
Hematological parameters of freshwater fish Piaractus mesopotamicus (Holmberg, 1887) kept in an intensive polyculture system were determined. The correlations among these parameters as well as those between the parameters and the biometric data (total weight and standard length) were also analyzed. Erythrocytes count was 1.708±0.42 x 10(6)/µl, hemoglobin 6.6±2.0 g% of blood and hematocrit 32.5±4.6%, mean corpuscular volume (MCV) 209.8±69.5%, mean corpuscular hemoglobin (MCH) 40.2±5.6 pg and mean corpuscular hemoglobin concentration (MCHC) 21.4±8.4%. The percentage of defense blood cells including leucocytes and thrombocytes, was studied. The percentage of thrombocytes was 61.4±10.0%, lymphocytes 21.7±1.8%,neutrophils 10.4±11.0%, monocytes 4.7±3.3% and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Piaractus mesopotamicus; Blood; Erythrocytes; Freshwater fish; Leucocytes; Thrombocytes.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751999000200008
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Blood biochemical profile of the South American rattlesnake (Crotalus durissus terrificus) in captivity J. Venom. Anim. Toxins
TROIANO,J. C.; GOULD,E. G.; ALTHAUS,R.; MALINSKAS,G.; GOULD,J. A.; HEKER,J.; VIDAL,J. C.; AMANTINI,E.; SIMONCINI,C..
Blood samples were collected from 180 healthy specimens of the South American rattlesnake, Crotalus durissus terrificus, in captivity. All animals were in good clinical condition. Normal biochemical reference values were established for the following: total proteins, albumin, globulins, uric acid, creatinine, urea, glucose, triglycerides, cholesterol, total lipids, calcium, phosphorus, potassium, sodium, chloride, magnesium, GOT (AST), GPT (ALT), and alkaline phosphatase (ALP). Samples were obtained by venipuncture of the ventral tail vein. Values were compared with published data for Boidae, Elapidae, and Viperidae.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood; Biochemistry Crotalus durissus terrificus captivity.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302001000200004
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Feed restriction on performance and blood parameters of broilers fed diets with different sodium levels Rev. Bras. Ciênc. Avic.
Junqueira,OM; Fonseca,LEC; Araújo,LF; Duarte,KF; Araújo,CS da S; Rodrigues,EAp.
The experiment was conducted to evaluate the effect of feed restriction and different sodium levels in the diet on the performance and hematological values (erythrocyte, hemoglobin, hematocrit, mean cell volume, mean cell hemoglobin, mean cell hemoglobin concentration and white blood cell) of broilers from 22 to 42 days of age. A completely randomized design was used in a 4 x 2 x 2 factorial (levels of sodium: 0.16%, 0.19%, 0.22% and 0.25%; with or without feed restriction; male and female), and two replicates of thirty broilers each. Sodium levels did not affect performance (p>0.05). Feed restriction resulted in increased feed intake and affected weight gain and feed conversion negatively (p<0.05). Better weight gain and feed conversion (p<0.05)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood; Broilers; Feed restriction; Nutrition; Sodium; Sodium bicarbonate.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2003000200003
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Egg traits and physiological neonatal chick parameters from broiler breeder at different ages Rev. Bras. Ciênc. Avic.
Luquetti,BC; Gonzales,E; Bruno,LDG; Furlan,RL; Macari,M.
This investigation was carried out to study egg traits (weight, specific gravity and eggshell thickness), and neonatal chick parameters (weight, blood, cardiac and lung parameters) from breeder at different ages. After hatching, neonatal chicks, male and female, from broiler breeder at three different ages (30, 45 and 60 weeks) were sacrificed and blood (red blood cell number, hematocrit, mean cell volume, blood viscosity, and haemoglobin), cardiac (right ventricle and total ventricle weights, cardiac index) and lung (mean pulmonary pressure, fresh relative lung weight) parameters were measured. No significant differences in eggshell thickness or specific gravity were observed in eggs from the three different breeder age. The incubated eggs and neonatal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broiler breeder age; Blood; Cardiac; Lung parameters; Neonatal chicks.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2004000100002
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Protective effect of sodium selenite on genotoxicity to human whole blood cultures induced by aflatoxin B1 BABT
Geyikoglu,Fatime; Türkez,Hasan.
The aim of this study was to investigate the effects of selenium and aflatoxin on human whole blood cultures (WBC) in relation to induction of sister-chromatid exchange (SCE). Results showed that the frequency of SCEs in peripheral lymphocytes was significantly increased by the direct-acting mutagen AFB1 (at doses 5 and 10 µM except for 1µM) compared with controls. When sodium selenite (Na2SeO3) was added at a molar ratio of 5x10-7 and 1x10-6, cells did not show significant increase in SCE frequency. Whereas, SCE rates induced by the various AFB1 concentrations could be significantly reduced by the presence of Na2SeO3 in a clear dose-related manner. These results indicated that selenite and AFB1 mutually antagonized their ability to cause DNA damage...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aflatoxin B1; Selenium; Sister-chromatid exchanges; Genotoxicity; Blood.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800006
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Studies on carbon-13 turnover in eggs and blood of commercial layers Rev. Bras. Ciênc. Avic.
Denadai,JC; Ducatti,C; Pezzato,AC; Carrijo,AS; Caldara,FR; Oliveira,RP.
This paper aimed at evaluating the influence of diets containing different isotopic values of carbon-13 turnover on the half-life of egg (yolk + albumen), yolk and albumen individually, and blood of poultry using delta‰13C isotopic variation. Commercial layers fed four experimental isocaloric and isonitrogenous diets (RC4, RC3, RMC4 and RMC3) containing different isotopic values, during an experimental period of 56 days. Turnover of the studied tissues was influenced by the experimental diets. Blood and albumen were more influenced by dietary treatments as compared to egg and yolk. The RMC3 diet induced better performance (better feed intake and higher egg production) due faster rate of carbon substitution than the RC4 diet, and lower half-life for egg...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Albumen; Carbon-13; Yolk; Blood; Egg; Comercial laying hens.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000400008
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Characterization of Blood Mononuclear Phagocytes in Phrynops hilarii (Chelonia Chelidae) International Journal of Morphology
Pitol,Dimitrius Leonardo; Issa,Joáo Paulo Mardegan; Caetano,Flávio Henrique; Lunardi,Laurelúcia Orive.
The localization of peroxidase activity in different cell regions is used as a criterion for the classification of the stage of maturation of mammalian mononuclear phagocytes with a positive peroxidase reaction indicating the presence of monoblasts, promonocytes, monocytes and macrophages. In this study it was evaluated the peroxidase activity of blood mononuclear phagocytes of this turtle detected at different stages of differentiation. The present observations suggest that, in turtles, the differentiation of mononuclear phagocytes occur in the blood circulation, in contrast to animals, where only are monocytes in circulating blood and macrophage differentiation occurs in other body compartments
Tipo: Journal article Palavras-chave: Mononuclear phagocytes; Blood; Turtle.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000200021
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Morphological Characterization of the Leukocytes in Circulating Blood of the Turtle (Phrynops hilarii) International Journal of Morphology
Pitol,Dimitrius Leonardo; Issa,Joao Paulo Mardegan; Caetano,Flávio Henrique; Lunardi,Laurelúcia Orive.
The Phrynops hilarii specie of turtle has its characterization not well defined in the literature, it was proposed in this study the leukocyte characterization of the blood, stained by Leishman and analyzed under light and transmission electron microscope. It was not observe any cellular type with similar characteristics to neutrophils in mammalian group. We believed, based on the data obtained in this study that the heterophils have a morphofuncional analogy with another neutrophils belonged to mammalian group. This conclusion is being supported in many recent studies found in the literature
Tipo: Journal article Palavras-chave: Turtle; Morphology; Leukocytes; Blood.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000400002
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Effect of a peel passion fruit flour (Passiflora edulis f. flavicarpa) extract on the labeling of blood constituents with technetium-99m and on the morphology of red blood cells BABT
Rebello,Bernardo Machado; Moreno,Silvana Ramos Farias; Ribeiro,Camila Godinho; Neves,Rosane de Figueiredo; Fonseca,Adenilson de Souza da; Caldas,Luis Querino de Araújo; Bernardo-Filho,Mario; Medeiros,Aldo da Cunha.
Passiflora edulis f. flavicarpa (maracuja) is a fruit consumed in Brazil and worldwide. Blood constituents labeled with technetium-99m (99mTc) are used in nuclear medicine. The effect of P. flavicarpa extract on the radiolabeling of blood constituents and on red blood cells morphology was evaluated. Blood samples from Wistar rats was incubated with P. flavicarpa extract. After that, the labeling of blood constituents with 99mTc was carried out. Samples of plasma and blood cells were precipitated with trichloroacetic acid to isolate the soluble and insoluble fractions of plasma and blood cells. The radioactivity in each fractions was counted and the percentage of radioactivity was determined. Blood smears were also prepared to morphological evaluation and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Labeling; Blood; Passiflora edulis f. flavicarpa; Radiobiocomplexes; Technetium-99m.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000600019
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Methodological limitations of counting total leukocytes and thrombocytes in reptiles (Amazon turtle, Podocnemis expansa): an analysis and discussion Acta Amazonica
Tavares-Dias,Marcos; Oliveira-Júnior,Antônio Aderson; Marcon,Jaydione Luiz.
The aim of this paper is to compare three different methods for counting white blood cells [WBC] (Natt and Herrick method, estimation with 1,000 and 2,000 erythrocytes) and three methods for counting total thrombocytes [TT] (Wojtaszek method, estimation with 1,000 and 2,000 erythrocytes) in a South American freshwater turtle species, Podocnemis expansa, Schweigger 1812 (Reptilia, Pelomedusidae). Direct WBC counts using the Natt and Herrick method showed limitations, which are discussed here. The WBC and TT counts using 1,000 erythrocytes from blood smears are not recommended for Amazon turtles nor other reptilian species, since wide variation in counts can be observed. Estimation methods for determining WBC and TT based on 2,000 erythrocytes of blood...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amazon turtles; Blood; Leukocytes; Podocnemis expansa; Thrombocytes.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672008000200020
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Comparative study on hematological parameters of farmed matrinxã, Brycon amazonicus Spix and Agassiz, 1829 (Characidae: Bryconinae) with others Bryconinae species Acta Amazonica
Tavares-Dias,Marcos; Affonso,Elizabeth Gusmão; Oliveira,Sarah Ragonha; Marcon,Jaydione Luiz; Egami,Mizue Imoto.
The aim of this study was not only to determine the red blood cells parameters, thrombocyte and leukocyte counts in farmed Brycon amazonicus (matrinxã), to compare these parameters among Bryconinae species from literature, and also to investigate the presence of special granulocytic cells in these fish. The results of the blood cells parameters here established for farmed B. amazonicus, a species of great economic importance in Brazilian aquaculture, could help a better understanding of the blood features in natural populations of this Amazon species. Blood parameters varied between Bryconinae species investigated, mainly the red blood cell counts, hemoglobin, hematocrit and mean corpuscular volume (MCV). The presence of the blood granulocytes, neutrophils...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leukocytes; Freshwater fish; Blood; Amazon; Brycon amazonicus; Bryconinae.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672008000400026
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Effects of fenoprofen on the labeling of blood constituents with technetium-99m, the morphology of red blood cells and the plasmid BABT
Pereira,Marcia de Oliveira; Rocha,Gabrielle de Souza; Lombardi,Simone dos Santos; Geller,Mauro; Pereira,Mário José; Santos-Filho,Sebastião David; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario.
The aim of this work was to evaluate the effect of fenoprofen on the labeling of blood constituents with technetium-99m, on the morphology of red blood cells and on the plasmid DNA. Blood samples from Wistar rats were incubated with fenoprofen and the assay of labeling of blood constituents with technetium-99m (99mTc) was performed. Blood cells, plasma, soluble and insoluble fractions of blood cells and plasma were separated. The radioactivity in each fraction was counted and percentage of incorporated radioactivity (%ATI) was determined. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of the red blood cells (RBC) was evaluated. Plasmid (pBSK) was incubated with fenoprofen with stannous chloride, and agarose gel...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Technetium-99m; Blood; Morphology; Plasmid; Fenoprofen.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700022
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Effect of an extract of Centella asiatica on the biodistribution of sodium pertechnetate (Na99mTcO4) and on the fixation of radioactivity on blood constituents BABT
Diniz,Claudia Leite; Carmo,Fernanda Santos do; Almeida,Danielle Santos de; Santos-Filho,Sebastião David; Missailidis,Sotiris; Fonseca,Adenilson de Souza da; Bernardo Filho,Mario.
This study evaluates the effects of an acute treatment with a Centella asiatica (CA) extract on the biodistribution of the radiopharmaceutical Na99mTcO4 and on the fixation of technetium-99m on blood constituents. Wistar rats were treated with CA extract and, 1 hour after, Na99mTcO4 was administered; organs/tissues were withdrawn and weighted. The radioactivity was counted to calculate the percentage of activity per gram (%ATI/g). Also, blood samples were withdrawn, plasma (P), blood cells (BC), insoluble fraction (IF) and soluble fractions of P and BC were isolated and the radioactivity was counted to calculate the percentage of activity (%ATI). Data indicated that the acute treatment with CA extract changed significantly (p<0.05) the %ATI/g in several...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodistribution; Centella asiatica; Blood; Plasma; Technetium-99m.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700035
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Effects of chronic sucralose sweetener on the labeling of blood constituents with technetium-99m, morphology of red blood cells and the biodistribution of sodium pertechnetate in rats BABT
Rocha,Gabrielle de Souza; Pereira,Marcia de Oliveira; Benarroz,Mônica de Oliveira; Frydman,Jacques Natan Grinapel; Garcia-Pinto,Angélica Beatriz; Pereira,Mário José; Fonseca,Adenilson de Souza da; Bernardo-Filho,Mario.
This work evaluates effects of the sweetener with sucralose on the labeling of blood constituents with technetium-99m (99mTc), on the morphology of red blood cells (RBC) and on the biodistribution of sodium pertechnetate in Wistar rats. Animals were treated with sweetener for 8 days. Blood samples were withdrawn and the assay of labeling of blood constituents with 99mTc was performed. Blood cells (BC) and plasma (P) were isolated. Aliquots of BC and P were also precipitated, soluble and insoluble fractions separated. The radioactivity in each fraction was counted and percentage of incorporated radioactivity (%ATI) determined. Blood smears were prepared, fixed, stained and the qualitative and quantitative morphology of the RBC was evaluated under optical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodistribution; Blood; Morphology; Sweetener; Technetium-99m.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700021
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Balanço eletrolítico e redução da proteína bruta da ração sobre parâmetros fisiológicos e sanguíneos de frangos de corte no calor - DOI: 10.4025/actascianimsci.v31i3.4792 Animal Sciences
Matos, Marize Bastos de; UENF; Ferreira, Rony Antonio; UNIVALE; Savaris, Vaneila Daniele Lenhardt; UENF; Couto, Humberto Pena; UENF; Soares, Rita da Trindade Ribeiro Nobre; UENF; Oliveira, Newton Tavares Escocard de; UENF.
Um experimento foi conduzido, objetivando-se avaliar o efeito do balanço eletrolítico (BER) e a redução da proteína bruta (PB) da ração sobre a frequência respiratória, temperatura cloacal, concentrações de Na+, K+, Cl- e ácido úrico em frangos de corte. Foram utilizados 800 pintos de corte, machos da linhagem Cobb, alojados em 20 boxes, distribuídos em delineamento inteiramente casualizado com quatro tratamentos, cinco repetições e 40 aves por unidade experimental. Os tratamentos foram: T1 - ração basal; T2 - ração corrigida para BER de 250 mEq kg-1; T3 - ração com redução de PB, sem correção de BER; T4 - ração com redução de PB corrigida para BER de 250 mEq kg-1. As condições ambientais evidenciaram que os animais estavam em estresse por calor. As aves...
Palavras-chave: 5.04.05.00-4 eletrólitos; Sangue; Homeostase; Estresse Ciencias Agrárias electrolytes; Homeostasis; Blood; Stress.
Ano: 2009 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/4792
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Procedimentos básicos para colheita de sangue em peixes. Infoteca-e
ISHIKAWA, M. M.; PÁDUA, S. B.; SATAKE, F.; PIETRO, P. S. de; HISANO, H..
O monitoramento do estado de saúde dos peixes é imprescindível para o manejo sanitário da produção, seja de grande ou pequeno porte. A hematologia clínica é uma ferramenta que permite a realização de diagnóstico de patologias e pode atuar como um indicador prognóstico das condições patológicas, especialmente quando se considera as alterações morfológicas nas células sanguíneas (SATAKE et al., 2009). Estas avaliações permitem identificar a resposta dos peixes, quando doentes, de maneira rápida, prática e de baixo custo
Tipo: Circular Técnica (INFOTECA-E) Palavras-chave: Manejo sanitário; Harvest.; Colheita; Peixe; Sangue.; Blood; Fish..
Ano: 2010 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/874164
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Light microscope observation of circulating human lymphocytes cultured in vitro BABT
Oliveira,Naila Francis Paulo de; Dolder,Mary Anne Heidi; Genari,Selma Candelária.
The purpose of this work was to study the isolation and a light microscopy technique for cultured lymphocytes. Blood samples were obtained by venipuncture with an anticoagulant added and centrifuged in a Percoll density gradient to separate the leukocytes. Lymphocytes were placed in 25 cm ³ tissue culture flasks at 37ºC. After culturing, they were fixed and stained with the methods used for blood smears. Results showed that not all fixing solutions and stains were an equally good choice for cultured lymphocytes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blood; In vitro; Lymphocytes; Microscopy; Morphology; Percoll.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500013
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Voluntary blood collection from the epidural sinus in two harbor seals (Phoca vitulina vitulina) OceanDocs
Sobrado, A..
La colección voluntaria de sangre en focas es una técnica díficil, el éxito depende de la habilidad del entrenador fundamentalmente. Requiere la relajación y cooperación total del animal. Se identifica aquí la secuencia de pasos que permiten esta actividad.
Tipo: Journal Contribution Palavras-chave: Haematology; Blood; Marine mammals.
Ano: 2011 URL: http://hdl.handle.net/1834/4163
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Produto à base de própolis (LLOS) na dieta de bovinos inteiros confinados: comportamento animal e respostas sanguíneas - doi: 10.4025/actascianimsci.v33i1.10671 Animal Sciences
Faria, Leonardo Avanzzi Nunes; UEM; Barbosa, Orlando Rus; UEM; Zeoula, Lucia Maria; UEM; Aguiar, Silvia Cristina de; UEM; Prado, Rodolpho Martin do; UEM; Bertolini, Dennis Armando; UEM.
Avaliou-se o comportamento, as respostas sanguíneas de animais confinados recebendo dieta com produto à base de própolis (LLOS). Os tratamentos foram: controle (CON) e dois produtos à base de própolis (LLOSC1) e (LLOSC1+). Mediram-se os comportamentos: comendo (COM), bebendo água (BEB), andando (AND), ruminando em pé (RUEMP), ruminando deitado (RUDET), em pé (EMP) e deitado (DET). Maior tempo em COM, BEB, AND, RUEMP e EMP ocorreu de manhã (MA) e à tarde (TA), e em RUDET e DET à noite (NO) e madrugada (MD). Animais no Lado 1 do confinamento permaneceram mais tempo andando na MA e à NO, sem diferenças para MD, e mais tempo EMP para os do Lado 1 na MA, T e MD, e igual à NO. Cortisol, Glicose e Ureia não foram influenciados pelos tratamentos. Não houve efeito...
Tipo: Pesquisa de campo Palavras-chave: 5.04.01.00-9 comportamento animal; Confinamento; Própolis; Sangue animal behavior; Feedlot; Propolis; Blood.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/10671
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